A REVIEW ON INTERPRETATION OF BIOLOGICAL VARIATIONS BETWEEN THE LOWRY VERSUS BRADFORD METHOD FOR PROTEIN QUANTIFICATION
Goutam Mukhopadhyay
M.R. College of Pharmaceutical Sciences and Research, Ashoknagar, Balisha, West Bengal 743234, India.
Shymodip Kundu
Department of Pharmaceutical Science & Technology, Maulana Abul Kalam Azad University & Technology, Nadia, Kolkata, West Bengal 700064, India.
Ivy Ghosh
M.R. College of Pharmaceutical Sciences and Research, Ashoknagar, Balisha, West Bengal 743234, India.
Ajay Kumar
Department of pharmacology, Government Pharmacy Institute, Agamkua, Patna, Bihar 800007, India.
Sourav Chatterjee
M.R. College of Pharmaceutical Sciences and Research, Ashoknagar, Balisha, West Bengal 743234, India.
Protein quantification is an essential component in many biological studies in evaluating quantitative protein expression. The identification of differences in protein expression resulting from methodical variations is an essential component to the interpretation of true, biologically significant results. In this study we compared Lowry and Bradford methods, two most commonly used methods for protein quantification, to assess whether differential protein expressions are a result of true biological or methodical variations. We analyzed differential protein expression patterns which were assessed by western blot following protein quantification by the Lowry and Bradford methods. The study showed significant variations in protein concentrations following assessment with the Lowry versus Bradford methods, using identical samples. Bradford method showed greater variations in protein concentration readings over time and in samples with higher concentrations. In this review article we have tried to show that methodical variations observed in these protein assay techniques, can potentially translate into differential protein expression patterns, which can be falsely taken to be biologically significant. Our study therefore highlights the pivotal need to carefully consider methodical approaches to protein quantification in techniques that report quantitative differences
11 , 2 , 2021
48 - 51